Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 15, Issue 4, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3470124
Keywords
biomedical optics; cells; detectors
Funding
- National Science Foundation [DBI-045468]
- National Institutes of Health [RO1-AI063366]
- Butcher Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0852863, 0852868] Funding Source: National Science Foundation
- Direct For Computer & Info Scie & Enginr
- Division Of Computer and Network Systems [1228778] Funding Source: National Science Foundation
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The measurement of cell elastic parameters using optical forces has great potential as a reagent-free method for cell classification, identification of phenotype, and detection of disease; however, the low throughput associated with the sequential isolation and probing of individual cells has significantly limited its utility and application. We demonstrate a single-beam, high-throughput method where optical forces are applied anisotropically to stretch swollen erythrocytes in microfluidic flow. We also present numerical simulations of model spherical elastic cells subjected to optical forces and show that dual, opposing optical traps are not required and that even a single linear trap can induce cell stretching, greatly simplifying experimental implementation. Last, we demonstrate how the elastic modulus of the cell can be determined from experimental measurements of the equilibrium deformation. This new optical approach has the potential to be readily integrated with other cytometric technologies and, with the capability of measuring cell populations, enabling true mechanical-property-based-cell cytometry. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3470124]
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