Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 14, Issue 3, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3130266
Keywords
endomicroscopy; microendoscopy; fluorescence; optical sectioning; fiber-bundle imaging
Funding
- NIH [R21 EB007338]
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We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an optically sectioned image with low resolution. This is complemented with high-resolution information from the uniform illumination image, leading to a full-resolution image that is optically sectioned. HiLo endomicroscope movies are presented of fluorescently labeled rat colonic mucosa. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3130266]
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