Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 13, Issue 4, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2968260
Keywords
two-photon absorption; excited state absorption (ESA); multiphoton microscopy; microvasculature imaging; oxygenation imaging
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The ability to perform high-resolution imaging of microvasculature and its oxygenation is very important in studying early tumor development. Toward this goal, we improved upon our excited state absorption (ESA)-based imaging technique to allow us to not only image hemoglobin directly but also differentiate between oxy- and deoxyhemoglobin in tissue. We demonstrate the separation of arterioles from venules in a live nude mouse ear using our imaging technique. (C) 2008 Society of Photo-Optical Instrumentation Engineers.
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