Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 13, Issue 2, Pages -Publisher
SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2904940
Keywords
tomography; Raman spectroscopy; bone; diffuse; imaging; non-invasive
Funding
- NCI NIH HHS [P01-CA080139, R01 CA120368, P01 CA080139-080004, R01-CA120368, P01 CA080139] Funding Source: Medline
- NIAMS NIH HHS [R01-AR055222, R01 AR055222, R01 AR055222-02] Funding Source: Medline
- NIDDK NIH HHS [T90 DK070071, T90-DK070071-03] Funding Source: Medline
- NIGMS NIH HHS [T32-GM008353, T32 GM008353, T32 GM145304] Funding Source: Medline
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Raman spectroscopic diffuse tomographic imaging has been demonstrated for the first time. It provides a noninvasive, label-free modality to image the chemical composition of human and animal tissue and other turbid media. This technique has been applied to image the composition of bone tissue within an intact section of a canine limb. Spatially distributed 785-nm laser excitation was employed to prevent thermal damage to the tissue. Diffuse emission tomography reconstruction was used, and the location that was recovered has been confirmed by micro-computed tomography (micro-CT) images. (C) 2008 Society of Photo-Optical Instrumentation Engineers.
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