Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 13, Issue 5, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.3000430
Keywords
optical projection tomography; confocal microscopy; pancreas; microbiopsy; insulitis; diabetes
Funding
- Swedish Research Council (Holmberg and Ahlgren)
- Juvenile Diabetes Foundation
- The Kempe Foundation, (Ahlgren)
- University Medical Faculty
- ICREA Funding Source: Custom
- MRC [MC_U127574434] Funding Source: UKRI
- Medical Research Council [MC_U127574434] Funding Source: researchfish
Ask authors/readers for more resources
A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required. (C) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3000430]
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available