Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 10, Issue 4, Pages 669-678Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2014.1625
Keywords
Silver; Nanoparticle; Chronic Myeloid Leukemia; Uptake; Cytotoxicity; Reactive Oxygen Species
Funding
- National Key Basic Research Program of China [2011CB933503, 2011CB933501]
- National Natural Science Foundation of China (NSFC) [61127002, 51201034, 31000453, 31200757, 31170959, 81000664]
- Special Project on Development of National Key Scientific Instruments and Equipment of China [2011YQ03013403]
- Natural Science Foundation of Jiangsu Province in China [BK2011036]
- Graduate Research and Innovation Program of Jiangsu Province in China [CXZZ-0172]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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Several studies have suggested that silver nanoparticles (AgNPs) have the potential to treat human cancers, including leukemia. However, the detailed cellular mechanisms for AgNPs to inhibit the growth of leukemic cells and their efficacy on clinical isolates of leukemic patients are not elucidated. In this study, the cellular uptake and cytotoxic mechanism of AgNPs in chronic myeloid leukemia (CML) cells were investigated. AgNPs were synthesized with a modified polyol method, which were stable under cell culture conditions with fetal bovine serum (FBS). AgNPs were demonstrated to be able to enter K562 cells (a CML cell line) in a dose-dependent manner and locate in endosomes. Reactive oxygen species (ROS) could be generated upon AgNPs exposure and cause cytotoxicity and apoptosis. It was also found that AgNPs treatment inhibited the viability of cells from CML patients (n = 4). The cell cycle status and several critical regulators were altered upon AgNPs treatment as well. All these cellular and molecular alterations caused by AgNPs exposure could be reversed by the addition of Vitamin C (an antioxidant). These results suggested that proper usage of AgNPs would be of great significance for CML treatment in future.
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