Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 10, Issue 6, Pages 1080-1090Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2014.1819
Keywords
Polycarprolactone; Alendronate; Nanofiber; Adipose-Derived Stem Cells; Bone Regeneration
Funding
- Korea Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea [HI11C0388]
- Korea Health Promotion Institute [HI11C0388010014] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2012M2A2A6013196] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The osteogenic effect of culturing adipose-derived stem cells (ADSCs) on alendronate (Aln)-loaded polycarprolactone (PCL) nanofibrous scaffolds was evaluated by examining alkaline phosphatase (ALP) activity, calcium content, and expression of osteogenic differentiation genes in vitro. The 10% Aln/PCL nanofibrous scaffolds showed more ALP activity, mineralization, and osteocalcin and osteopontin mRNA than the 1% or 5% Aln/PCL nanofibrous scaffolds. The capacity of Aln/PCL nanofibrous scaffolds to regenerate new bone was studied in a rat calvarial defect model. New bone formation in vivo was evaluated by radiography, micro-computed tomography, and histological analysis. At 8 weeks after implantation, Aln/PCL scaffolds had a positive effect on bone regeneration and matrix formation. These results suggested that Aln/PCL nanofibrous scaffolds enhanced the osteogenic differentiation of ADSCs in vitro and bone formation in vivo.
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