Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 8, Issue 5, Pages 786-790Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2012.1446
Keywords
Chemiluminescence; HBV-DNA; Hybridization; Magnetic Nanoparticles
Funding
- National Key Special Program of Science and Technology Infrastructure Program [2009ZX10004-311]
- Nanjing Longliang Biological Science and Technology Limited Company
- State Key Laboratory of Bioelectronics, Southeast Universitypound [2011E11]
- China Postdoctoral Science Foundation [2011M500841]
- Jiangsu Planned Projects for Postdoctoral Research Funds [1102005C]
- Hunan Science and Technology Project [2010PP4002]
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Hepatitis B virus is a kind of DNA virus which can cause serious epidemic disease. The analysis and detection of sequence-specific DNA have great significance in forensic analysis, early-stage identification and treatment of genetic disorders. Chemiluminescent detection of DNA has been applied in many fields, such as biological technology and molecular biology, due to its simple operation and high sensitivity. On the other hand, owing to possessing easy magnetic separation and large surface properties, magnetic nanoparticles have also been employed as special carriers to immobilize biomolecules. In this paper, the magnetic nanoparticles are prepared by soft-template method with uniform shape and good dispersion. Then a detection method of hepatitis B virus DNA is established taking advantages of both chemilumiescence with the system of alkaline phosphatase catalyzing 3-(2'-spiroadamantane)- 4-methoxy -4-(3 ''-phosphoryloxy) phenyl-1, 2-dioxetane and magnetic nanoparticles. The optimization of conditions affecting the hybridization reaction and the chemilumiescence detection are also investigated to promise a high sensitivity.
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