Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 7, Issue 1, Pages 98-99Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2011.1220
Keywords
Zinc Oxide; Nanoparticle; Reactive Oxygen Species; DNA Damage
Funding
- CSIR, New Delhi [NWP35, SIP-08]
- DST-NSTI [SR/S5/NM-01/2007]
- UK India Education and Research Initiative (UKIERI) [SA 07-067]
- Council of Scientific and Industrial Research (New Delhi)
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Zinc oxide (ZnO) is being used worldwide in consumer products and industrial applications. As humans are being directly exposed to ZnO nanoparticles (NPs) through different routes, it is likely that the NPs would gain access to the liver. Therefore, the present study investigated the cytotoxic and genotoxic potential of ZnO nanoparticles in human liver cells (HepG2). The MIT and neutral red uptake assay showed a significant (p < 0.05) concentration and time dependent toxicity after 12 and 24 h at 14 and 20 mu g/ml. A (p < 0.05) significant increase in DNA damage was observed in cells exposed to ZnO NPs for 6 h as evident with an increase in the Olive tail moment (OTM) and % tail DNA in the Comet assay. The generation of intracellular reactive oxygen species further suggest the role of oxidative stress in ZnO nanoparticle mediated DNA damage and cytotoxicity in HepG2 cells.
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