Different Active Biomolecules Involved in Biosynthesis of Gold Nanoparticles by Three Fungus Species

In this paper, the intracellular gold nanoparticles were biosynthesized using three fungi including Aureobasidium pullulans (A. pullulans), Fusarium sp. and Fusarium oxysporum (F oxysporum) after immersion the fungal cells in AuCl4- ions solution. UV-vis and FTIR spectrum, and biochemical compositions analysis of Au nano-fungal cells suggested that active biomolecules of reducing sugar of A. pullulans, and proteins in Fusarium sp. and F oxysporum were tested positive of providing the function of the reduction of AuCl4- ions and the formation of the gold crystals. SDS-PAGE analysis of purified protein from gold nanoparticles synthesized by three fungi showed that three proteins with molecular weight (WM) about 100 kDa, 25 kDa and 19 kDa were in the gold nanoparticles by Fusarium sp. and two proteins with WM about 25 kDa and 19 kDa were in gold nanopartides of F oxysporum. Further, three purified fungal proteins with WM about 100 kDa, 25 kDa and 19 kDa from gold nanoparticles by Fusarium sp. identified by LC-MS/MS, named plasma membrane ATPase, 3-glucan binding protein and glyceraldehyde-3-phosphate dehydrogenase, respectively. The Au nano-fungal cells ultrathin sections of Fusarium sp. and F oxysporum showed that the gold nanoparticles mainly produced in intracellular vacuoles of fungal cells. The growth of gold nanopartides in three fungal cells indicated the reducing sugar led to the gold nanoparticles in spherical morphology and proteins benefited to the gold aggregates.