Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 95B, Issue 1, Pages 192-201Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.b.31702
Keywords
coating; osteogenesis; plasma spray; CaO-ZrO2-SiO2; human bone marrow-derived stromal cells
Funding
- National Natural Science Foundation of China [30600628]
- National High Technology Research and Development Program of China [2006AA02A137]
- Shanghai Rising-Star Program [08QA14045]
- Program for Shanghai Key Laboratory of Orthopaedic Implant [08DZ2230330]
- Fund for Key Disciplines of Shanghai Municipal Education Commission [J50206]
- National Basic Research Program [2010CB945600]
- National Science and Technology Project [2009ZX09503-024]
- Key Projects in the National Science and Technology Pillar Program [2006BAI16B01]
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The CaO-ZrO2-SiO2 (CZS) coating was prepared by plasma-spraying chemically synthesized CZS powder onto a Ti-6-AI-4V substrate. This CZS coating has been demonstrated to have good bioactivity, high bonding strength with the substrate and a low degradation rate. However, the effect of CZS coating on the osseointegration of bone-implant is still unknown. In this study, human bone marrow-derived stromal cells (hBMSCs) were cultured on CZS coating in vitro, and cell behavior was investigated, with the classical hydroxyapatite (HA) coating as a control. Scanning electron microscopy (SEM) and immunofluorescence studies showed that the hBMSCs on the CZS coating spread well with organized cytoskeleton structure at 24 h following cell seeding. The MU assay and the Ala mar Blue assay indicated that CZS coating promoted the attachment and proliferation of hBMSCs. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes, such as ALP, collagen I (COLI), osteopontin (OPN), and osteocalcin (OCN), demonstrated that the osteoblastic differentiation of hBMSCs was enhanced more by CZS coating than by HA coating. These results suggest that CZS coating possess excellent biological properties and may have potential in biomedical applications. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 95B: 192-201,2010.
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