Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 95B, Issue 1, Pages 110-117Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.b.31689
Keywords
spinal cord injury; hyaluronic acid scaffold; nogo-66 receptor antibody; adhesion; poly-(L)-lysine
Funding
- National Basic Research Program of China [2005CB623905, 2007CB947704]
- National Natural Science Foundation of China [30670656, 50973052]
- National Natural Science Foundation of Beijing [705001]
- Beijing Scientific Committee [20005187040311]
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The biomaterials used for central nervous system injury require not only interacting with specific cell adhesion but also specific growth factor receptors to promote nerve regeneration. In this study, hyaluronic acid (HA)-based hydrogels modified with poly-L-lysine (PLL) and nogo-66 receptor antibody (antiNgR) (HA-PLL/antiNgR) were administered to rats after lateral hemisection of the spinal cord. Anti-neurofilament positive axons were found to extend into the HA-PLL/antiNgR hydrogel at 8 weeks after implantation, which shows significant difference compared with HA-PLL or blank control group. Electron micrographs of implanted hydrogels showed that there were more cells and normal axons with myelin in the HA-PLL/antiNgR implant than that of HA-PLL hydrogel. The antiNgR grafted on HA hydrogels could be detected for 8 weeks after transplantation in vivo. All of these properties may facilitate HA-PLL/antiNgR hydrogels to become a promising scaffold for repairing spinal cord injury. Nevertheless, both two kinds of modified hydrogels (HA-PLL/antiNgR and HA-PLL) showed remarkable advantages in supporting angiogenesis, and simultaneously inhibiting the formation of glial scar. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 95B: 110-117, 2010.
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