Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 91B, Issue 1, Pages 366-372Publisher
WILEY
DOI: 10.1002/jbm.b.31411
Keywords
platelet-derived growth factor (PDGF); demineralized bone matrix (DBM); heparin; cellularization; vascularization
Funding
- NSFC [30688002, 30600304]
- Ministry of Science and Technology of China [2006CB943601]
- Chinese Academy of Sciences [KSCX2-YW-R-133]
- K. C. Wong Education Foundation
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Platelet-derived growth factor (PDGF) plays an important role in tissue regeneration and wound repair. However, the lack of effective delivery and the efficient targeting specificity limits its clinical applications. Here, heparin possessing PDGF binding domain was crosslinked to the collagen-based demineralized bone matrix (DBM) for the delivery of human PDGF(HC-PDGF). In in vitro experiments, heparin improves the binding of PDGF to collagen. lit vitro activity assay indicates that collagen-heparin-PDGF (CH-PDGF) promotes human fibroblasts to proliferate on collagen gel. In addition, HC-PDGF stimulates cells to migrate into DBM scaffolds after implantation. The histological analysis shows that HC-PDGF promotes vascularization of the implants. In summary, heparin-DBM/PDGF could prevent the diffusion of PDGF, prolong its activity, and promote the cellularization and vascularization of the scaffold. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 91B: 366-372 2009
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