Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Volume 87B, Issue 2, Pages 415-424Publisher
WILEY-LISS
DOI: 10.1002/jbm.b.31119
Keywords
autologous chondrocytes in artificial scaffold; cartilage regeneration; fibrin; hyaluronan; collagen
Funding
- Internal Grant Agency of the Ministry of Health of the Czech Republic (IGA) [8122-3/2004]
- Grant Agency of the Czech numbers [1ET-400110403, 304/08/0256]
- Centre for Cardiovascular Research [IM6798582302]
- Grant Agency of the Charles University [121/2005/B-BIO/2.LF]
- [AV0Z50390512]
- [AV0Z 50110509]
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A new composite scaffold containing type I collagen, hyaluronan, and fibrin was prepared with and without autologous chondrocytes and implanted into a rabbit femoral trochlea. The biophysical properties of the composite scaffold were similar to native cartilage. The macroscopic, histological, and immunohistochemical analysis of the regenerated tissue from cell-seeded scaffolds was performed 6 weeks after the implantation and predominantly showed formation of hyaline cartilage accompanied by production of glycosaminoglycans and type 11 collagen with minor fibro-cartilage production. Implanted scaffolds without cells healed predominantly as fibro-cartilage, although glycosaminoglycans and type 11 collagen, which form hyaline cartilage, were also observed. On the other hand, fibro-cartilage or fibrous tissue or both were only formed in the defects without scaffold. The new composite scaffold containing collagen type 1, hyaluronan, and fibrin, seeded with autologous chondrocytes and implanted into rabbit femoral trochlea, was found to be highly effective in cartilage repair after only 6 weeks. The new composite scaffold can therefore enhance cartilage regeneration of osteochondral defects, by the supporting of the hyaline cartilage formation. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 87B: 415-424, 2008
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