Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 103, Issue 3, Pages 919-928Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.35234
Keywords
electroactive polymer; stem cell; cell adhesion; osteogenesis
Funding
- Academy of Finland [136288, 140978, 256931]
- Sigrid Juselius Foundation
- Pirkanmaa Hospital District
- Finnish Funding Agency for Technology and Innovation (TEKES)
- FEDER through the COMPETE Program
- Portuguese Foundation for Science and Technology (FCT) [PEST-C/FIS/UI607/2011, NANO/NMed-SD/0156/2007, PTDC/CTM-NAN/112574/2009]
- Project Matepro-Optimizing Materials and Processes
- Programa Operacional Regional do Norte (ON.2-O Novo Norte), under the Quadro de Referencia Estrategico Nacional (QREN), through the Fundo Europeu de Desenvolvimento Regional (FEDER) [NORTE-07-0124-FEDER-000037]
- FCT [SFRH/BPD/63148/2009, SFRH/BPD/90870/2012]
- Academy of Finland (AKA) [140978, 256931, 256931] Funding Source: Academy of Finland (AKA)
- Fundação para a Ciência e a Tecnologia [NANO/NMed-SD/0156/2007, PTDC/CTM-NAN/112574/2009] Funding Source: FCT
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Poly(vinylidene fluoride) (PVDF) is a biocompatible material with excellent electroactive properties. Nonelectroactive -PVDF and electroactive -PVDF were used to investigate the substrate polarization and polarity influence on the focal adhesion (FA) size and number as well as on human adipose stem cells (hASCs) differentiation. hASCs were cultured on different PVDF surfaces adsorbed with fibronectin and FA size and number, total adhesion area, cell size, cell aspect ratio and FA density were estimated using cells expressing vinculin fused to enhanced green fluorescent protein. Osteogenic differentiation was also determined using a quantitative alkaline phosphatase assay. The surface charge of the poled PVDF films (positive or negative) influenced the hydrophobicity of the samples, leading to variations in the conformation of adsorbed extracellular matrix proteins, which ultimately modulated the stem cell adhesion on the films and induced their osteogenic differentiation. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 919-928, 2015.
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