Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 102, Issue 9, Pages 3102-3111Publisher
WILEY
DOI: 10.1002/jbm.a.34983
Keywords
adipose-derived stem cells; SPCL; calvarial defect; critical size defect; bone regeneration
Funding
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/44128/2008, MIT/ECE/0047/2009]
- COBRE [NIH-8 P20 GM103528]
- NORC [NIH 1P30-DK072476]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/44128/2008, MIT/ECE/0047/2009] Funding Source: FCT
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The repair of large bony defects remains challenging in the clinical setting. Human adipose-derived stromal/stem cells (hASCs) have been reported to differentiate along different cell lineages, including the osteogenic. The objective of the present study was to assess the bone regeneration potential of undifferentiated hASCs loaded in starch-polycaprolactone (SPCL) scaffolds, in a critical-sized nude mice calvarial defect. Human ASCs were isolated from lipoaspirate of five female donors, cryopreserved, and pooled together. Critical-sized (4 mm) calvarial defects were created in the parietal bone of adult male nude mice. Defects were either left empty, treated with an SPCL scaffold alone, or SPCL scaffold with human ASCs. Histological analysis and Micro-CT imaging of the retrieved implants were performed. Improved new bone deposition and osseointegration was observed in SPCL loaded with hASC engrafted calvarial defects as compared to control groups that showed little healing. Nondifferentiated human ASCs enhance ossification of nonhealing nude mice calvarial defects, and wet-spun SPCL confirmed its suitability for bone tissue engineering. This study supports the potential translation for ASC use in the treatment of human skeletal defects. (C) 2013 Wiley Periodicals, Inc.
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