Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 100A, Issue 8, Pages 1963-1971Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34052
Keywords
alginate; oxygen; perfluorocarbon; cellular encapsulation
Funding
- Diabetes Research Institute Foundation
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Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg-PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg-PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg-PFC hydrogel beads fabricated via Ba2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg-PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg-PFC hydrogels retain some of the PFC's biological-relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three-dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.
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