Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 101, Issue 2, Pages 413-420Publisher
WILEY
DOI: 10.1002/jbm.a.34339
Keywords
biocompatibility; endothelialization; layer-by-layer; stent; heparin
Funding
- National Key Technology R & D Program of China [2012 BAI18B02]
- National Key Basic Research Program of China [2012 CB619101]
- Chongqing City [CSTC 2010 AB5116]
- Fundamental Research Funds for the Central Universities [CDJZR 11230010, CDJXS 11230008]
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In-stent restenosis and subsequent thrombosis remain a significant complication following the implantation of coronary stents. Different approaches have been used in developing novel coronary stents to protect against thrombosis and minimize restenosis. In the present study, we designed a biomacromolecular layer-by-layer coating with heparin, vascular endothelial growth factor (VEGF), and fibronectin onto nickel-free titanium surface to improve blood compatibility and endothelial cell proliferation. The multilayer assembling process was monitored by water contact angle and surface plasmon resonance, respectively. With increasing the number of layers, the deposition of polyelectrolyte as self-assembled ultrathin multilayer films showed linear growth of absorbance. In vitro blood compatibility results revealed that the fabricated layers prolonged activated partial thrombin time and prothrombin time, reduced platelets activation and aggregation, and reduced blood hemolysis rate. Cell adhesion and growth results showed that the assembled multilayer films significantly promoted cell attachment and growth, and the endothelialization property of the multilayer films was preferable compared with the untreated titanium disk. In conclusion, these results suggest that titanium surface modification using biofunctional multilayer films composed of heparin, VEGF, and fibronectin may serve as a potential approach to inhibit thrombosis and promote re-endothelialization of cardiovascular stents. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
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