Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 100A, Issue 6, Pages 1467-1476Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34051
Keywords
drug delivery systems; alginate; albumin; nanoparticles; tamoxifen; cell cultures
Funding
- Ministerio de Ciencia e Innovacion of Spain [FIS PS09/01513, MAT2010-21509-C03-03]
- Universidad Complutense de Madrid (UCM) Grupos UCM [920613]
- UCM (FPI)
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Nanoparticles based on disulfide bond reduced bovine serum albumin and thiolated alginate (alginate-cysteine conjugate) have been prepared by coacervation method and have been loaded with tamoxifen (TMX). The TMX load into the nanoparticles was optimized (46 mu g/mg NP) by freeze-drying the systems before the loading procedure. Maximum TMX release (4552%) took place between 2 and 25 h. Cytotoxicity of unloaded nanoparticles in MCF-7 and HeLa cells was not observed, although a small decrease in viability took place at very high concentration. Cell uptake of nanoparticles occurred in both cell types and the presence of polysaccharide in the nanoparticle composition allowed a better interaction with cells. The administration of 10 mu M TMX by TMX-nanoparticles was effective in both cellular lines, and the effect of the drug-loaded systems on MCF-7 cell cycle showed the efficacy of the TMX-loaded nanoparticles. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
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