Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 100A, Issue 11, Pages 3001-3007Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34231
Keywords
human osteoblast; graphene; fetal bovine serum
Funding
- Academy of Sciences of the Czech Republic [IAA400400911, KAN400100701, KAN200100801, KAN115600801]
- Czech Grant Agency [P204/10/1677, P208/12/1062]
- Czech Ministry of Education, Youth and Sports [ME9060, MSM 0021620806]
- Charles University, 1st Faculty of Medicine [PRVOUK-P-24/LF1/3, UNCE - 204011]
- Charles University, Faculty of Science [GAUK: 443211, SVV-2011-263206]
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The influence of single-layer graphene produced by chemical vapor deposition on human osteoblast cells under different conditions was studied. Measurements probed the ability of cells to adhere and proliferate on graphene compared with SiO2/Si substrates and standard tissue culture plastic when cells were incubated for the first 2 h in the presence or the absence of fetal bovine serum (FBS), thus influencing the initial, direct interaction of cells with the substrate. It was found that after 48 h of human osteoblast incubation on graphene films, there were a comparable number of cells of a similar size irrespective of the presence or the absence of serum proteins. On the other hand, a strong initial influence through the presence of FBS proteins on cell number and cell size was observed in the case of the SiO2/Si substrate and control plastic. Thus, our study showed that the initial presence/absence of FBS in the medium does not determine cell fate in the case of a graphene substrate, which is very unusual and different from the behavior of cells on other materials. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:30013007, 2012.
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