Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 96A, Issue 3, Pages 513-519Publisher
WILEY
DOI: 10.1002/jbm.a.32999
Keywords
nonionic surfactant; synthetic vesicle; noisome; polysorbates; gene delivery
Funding
- NIH [CA114612, NS066945]
- WCU project of South Korea [R31-2008-000-10103-01]
- Chinese Academy of Sciences
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Synthetic nonionic surfactant vesicles (niosomes) are a colloidal system with closed bilayer structures, displaying distinct advantages in stability and cost compared with liposomes. In this article, polysorbate cationic niosomes (PCNs) were developed as gene carriers. The PCNs comprised nonionic surfactants (i.e., polysorbates) and a cationic cholesterol, and were synthesized using a film hydration method. The niosomes thus prepared possessed a regular morphology, and a particle size of 100 similar to 200 nm, and a zeta potential of +30 similar to 45 mV. The PCNs showed great physical stability over the course of 4 weeks at room temperature. The binding capacity of PCNs toward oligo-deoxynucleotides (ODN) was assessed by a gel retardation approach, which demonstrated that the ionic complexes were formed when +/- charge ratio reached to 4 or greater. Gene transfer study showed that the PCNs exhibited a high efficiency in mediating cellular uptake and transferred DNA expression. Based on these findings, PCNs may offer the potential to function as an effective gene delivery system. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 513-519, 2011.
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