Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 97A, Issue 3, Pages 272-280Publisher
WILEY
DOI: 10.1002/jbm.a.33054
Keywords
silk; fibroin; nanofiber; hydroxyapatite; osteoblast
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Shinshu University
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In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 97A: 272-280, 2011.
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