Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 99A, Issue 2, Pages 231-239Publisher
WILEY
DOI: 10.1002/jbm.a.33180
Keywords
different types of carbon nanotubes; polyamidoamine dendrimer-functionalization; GFP gene; transfection efficiency; cytotoxicity
Funding
- National Natural Science Foundation of China [20705008, 20875029, 20905025]
- Hunan Provincial Natural Science Foundation of China [09JJ6026]
- Hunan Normal University [ET20804]
- Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
- Hunan Provincial Education Department [09B062]
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Three types of functionalized carbon nanotubes (f-CNTs), polyamidoamine (PAMAM) dendrimer-functionalized single and multi-walled CNTs (MWCNT-PAMAM-1, MWCNT-PAMAM-2, and SWCNT-PAMAM-3), were prepared by covalent linkage approach. The micro-morphologies of the three f-CNTs and the interaction of MWCNT-PAMAM-2 with HeLa cells were characterized by transmission electron microscopy (TEM). The free amine groups on the surface of the three types of CNTs-PAMAM hybrids were quantitatively analyzed. Their cytotoxicity and transfection efficiency of plasmid DNA of enhanced green fluorescent protein (pEGFP-N1) to HeLa cells were investigated in detail. The results suggest that although all three types of CNTs-PAMAM hybrids can deliver pEGFP-N1 into HeLa cells and the exogenous GFP gene has been successfully expressed, MWCNT-PAMAM-2 with shorter length and larger amount of free amine groups on its surface possesses higher transfection efficiency (6.79%), being about 3.0 and 1.7 times as large as those of MWCNT-PAMAM-1 (2.24%) and SWCNT-PAMAM-3 (4.08%), respectively; their cytotoxicity to HeLa cells decrease following the sequence of SWCNT-PAMAM-3 > MWCNT-PAMAM-2 > MWCNT-PAMAM-1. These results may be useful for understanding the effects of the chemical/physical properties of f-CNTs on their gene transfection efficiency and cytotoxicity, allowing for construction of promising CNT-based intracellular delivery vectors for gene therapy. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 231-239, 2011.
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