Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 96A, Issue 1, Pages 221-229Publisher
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.32976
Keywords
titanium dioxide nanoparticles; mice; liver; inflammatory response; signaling pathway
Funding
- National Natural Science Foundation of China [30901218]
- Bringing New Ideas Foundation of postgraduate of Medical College of Soochow University
- Medical Development Foundation of Soochow University [EE120701]
- National Bringing New Ideas Foundation of Student of China [57315427, 57315927]
Ask authors/readers for more resources
In an effort to examine signaling pathway of inflammation of the mouse liver caused by intragastric administration of titanium dioxide nanoparticles (NPs), we assessed Toll-like receptor-2 (TLR2), TLR-4, I kappa B kinase (IKK-alpha, IKK-beta), I kappa B nucleic factor-kappa B (NF-kappa B), NF-kappa BP52, NF-kappa BP65, tumor necrosis factor-alpha (TNF-alpha), NF-kappa B-inducible kinase (NIK), interleukin-2 (IL-2), biochemical parameters of liver functions, and histopathological changes and liver ultrastructure in the TiO2 NPs-treated mice. The results showed the titanium accumulation in liver, histopathological changes and hepatocytes apoptosis of mice liver, and the liver function damaged by TiO2 NPs. The real-time quantitative reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay analyses showed that TiO2 NPs can significantly increase the mRNA and protein expression of TLR2 and TLR4 and several inflammatory cytokines, including IKK1, IKK2, NF-kappa B, NF-kappa BP52, NF-kappa BP65, TNF-alpha, and NIK, and TiO2 NPs can significantly decrease the mRNA and protein expression of I kappa B and IL-2. The results of this study added to our understanding of TiO2 NPs-induced liver toxicity. It implied that the signaling pathway of liver injury in the TiO2 NPs-stimulated mouse liver sequentially might occur via activation of TLRs -> NIK -> I kappa B kinase -> NF-kappa B -> TNF-alpha -> inflammation -> apoptosis -> liver injury. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 221-229, 2011.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available