Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 96A, Issue 1, Pages 204-211Publisher
WILEY
DOI: 10.1002/jbm.a.32975
Keywords
genipin; gelatin; microcarrier; liver; HepG2 cells; tissue engineering
Funding
- Ministry of Education (MoE), Singapore [AcRF Tier 1 RG 64/08]
- National Medical Research Council, Singapore [NMRC/EDG/1001/2010]
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In engineered regenerative medicine, various types of scaffolds have been customized to pursue the optimal environment for different types of therapeutic cells. In liver therapeutic research, hepatocytes require attachment to solid anchors for survival and proliferation before they could grow into cellular aggregates with enhanced functionalities. Among the various biomaterials scaffolds and vehicles, microspherical cell carriers are suited to these requirements. Individual spheres may provide two-dimensional (2D) cell-affinitive surfaces for cell adhesion and spreading; whereas multiple microcarriers may form three-dimensional (3D) matrices with inter-spherical space for cell expansion and multicellular aggregation. In this study, we culture human liver carcinoma cell line (HepG2) cells on genipin-crosslinked gelatin microspheres of two different sizes. Results suggest that both microcarriers support cell adhesion, proliferation, and spontaneous formation of hepatocellular aggregates, among which the spheres with bigger size (200-300 mu m) seem more favorable than the smaller ones in terms of aggregate formation and liver specific functionalities. These findings suggest that the genipin-crosslinked microcarrier is a competent vehicle for liver cell delivery. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 204-211, 2011.
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