Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 96A, Issue 2, Pages 402-412Publisher
WILEY
DOI: 10.1002/jbm.a.32995
Keywords
material; species; multinucleated cell; osteoclast; osteoinduction
Funding
- Japanese Ministry of Education, Science, Sports and Culture [17591566]
- Grants-in-Aid for Scientific Research [17591566] Funding Source: KAKEN
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Material-induced osteoinduction is reported in comparatively large animals such as dogs and pigs; however, it does not often occur in small animals such as rodents. In this study, we implanted porous calcium-deficient hydroxyapatite (CDHA) in the dorsal muscles of dogs and rats and compared the two species, with emphasis on multinucleated cells, by using hematoxylin and eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, transmission electron microscope (TEM) observation, and reverse transcription-polymerase chain reaction (RT-PCR). In CDHA extracted from dogs, numerous TRAP-positive multinucleated cells were detected after 2 weeks and new bone formation was observed after 4 weeks. In contrast, in rats, only a small number of TRAP-positive cells were detected and no bone formation was observed within 6 weeks. CDHA was more degraded in dogs than in rats. TEM observation of the multinucleated cells in CDHA extracted from dogs after 3 weeks revealed osteoclast-like features such as ruffled borders. However, CDHA extracted from rats did not exhibit osteoclast-like features. RT-PCR evaluation showed that the expression of cathepsin K was higher in dogs than in rats. These results indicate that TRAP-positive cells might be one of the main factors responsible for the cross-species difference in material-induced osteoinduction. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 402-412, 2011.
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