Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 94A, Issue 1, Pages 27-37Publisher
WILEY
DOI: 10.1002/jbm.a.32654
Keywords
biodegradable polymers; KGF; aerosol-OT; drug release; ToF-SIMS
Funding
- NSF [CHE-061696]
- NIH [5R13DK69503-3]
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Biodegradable polymers are of interest for developing controlled protein drug delivery platforms. in this study, two poly (alpha-hydroxy) esters were formulated with Aerosol-OT, a surfactant stabilizer, to encapsulate the protein keratinocyte growth factor (KG F) for controlled release KGF is involved in a number of crucial biologic processes, most notably epithelial growth and repair. The concentration of KGF that caused a biological response in vitro was determined (optimally 10 ng/mL) and compared with the release of KGF from the two biodegradable polymer membrane formulations. Each polymer formulation released biologically relevant levels, 10 ng/mL of active KG F, although with different times release kinetics. The membrane composed of PLGA/AOT/KGF exhibited a faster release rate of KGF into solution after 120 h of degradation time than the release rate of the PLLA/AOT/KGF matrices. Cell seeding assays showed that both polymer matrices, when formulated with AOT, sustained cell growth. Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) was used to characterize the distribution of AOT and KGF through the polymer membrane. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res 94A: 27-37, 2010
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