Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 89A, Issue 4, Pages 929-941Publisher
WILEY
DOI: 10.1002/jbm.a.32044
Keywords
adipose tissue engineering; scaffold; extracellular matrix; hyaluronan; stem cells
Funding
- Utah Centers of Excellence Program, Natural Sciences and Engineering Research Council (NSERC) of Canada
- Canadian Institutes of Health Research (CIHR), the Province of Ontario
Ask authors/readers for more resources
Placental decellular matrix (PDM) and PDM combined with cross-linked hyaluronan (XLHA) scaffolds, seeded with primary human adipose-derived stem cells (ASC), were investigated in a subcutaneous athymic mouse model. The in vivo response at 3 and 8 weeks was characterized using histological and immunohistochemical staining. Fibrous capsule formation was assessed and the relative number of adipocytes in each scaffold was quantified. Undifferentiated ASC were localized using immunostaining for human vimentin. Unilocular and multilocular adipocytes were identified by intracellular lipid accumulation. Staining for murine CD31 assessed implant vascularization. Both scaffolds macroscopically maintained their three-dimensional volume and supported mature adipocyte populations in vivo. There was evidence of implant integration and a host contribution to the adipogenic response. The results Suggested that incorporating the XLHA had a positive effect in terms of angiogenesis and adipogenesis. Overall, the PDM and PDM with XLHA scaffolds showed great promise for adipose tissue regeneration. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 89A: 929-941, 2009
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available