Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 93A, Issue 3, Pages 1068-1079Publisher
WILEY
DOI: 10.1002/jbm.a.32607
Keywords
block copolymers; molecular imaging; cytotoxicity; nanoparticles; two-photon absorbing materials
Funding
- NIH (Microscale Life Sciences Center (MLSC)-A Center of Excellence in Genome Sciences)
- Boeing-Johnson Foundation
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [P50HG002360] Funding Source: NIH RePORTER
Ask authors/readers for more resources
A hydrophobic two-photon absorbing (2PA) red emitter (R) was successfully incorporated into micelles formed from two block copolymers, poly(epsilon-caprolactone)block-poly(ethylene glycol)s, for imaging and toxicity studies. In micelles, the chromophore R exhibits a 2PA cross-section of 400 GM (1 GM = 1 x 10(-50) cm(4) s photon(-1) molecule(-1)) at 820 nm, which is among the highest values reported for red 2PA emitters. The micelles with a cationic amino moiety-containing poly(ethylene glycol) corona showed an enhancement of cell internalization and delivered the dye into the cytoplasmic regions of the mouse macrophage RAW 264.7 cells. In comparison, the dye in micelles with neutral poly(ethylene glycol) as corona could not be delivered into the cells. Cytotoxicity of the micelle-R constructs was studied using a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. More than 90% of the cells were viable after they were stained with the dye-containing micelles at different concentrations (dye concentrations of 2-6 mu M and polymer concentrations of 0.05-0.15 mg/mL) for 16 h. This is the first reported application of a hydrophobic 2,1,3-benzothiadiazole-containing 2PA red emitter delivered into the cytoplasm of cells for bioimaging and toxicity assessment. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 1068-1079, 2010
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available