Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 88A, Issue 1, Pages 174-183Publisher
WILEY
DOI: 10.1002/jbm.a.31884
Keywords
BMP-2; DMCB; heparin mimetic; interaction between BMP-2 and DMCB; biological activity
Funding
- Ministere de la Recherche [MJENR 02130212]
- Centre National de la Recherche
- Fondation pour la Recherche Medicale of France
Ask authors/readers for more resources
In the present study, we explored the binding capacity of synthetic heparin-like dextran derivatives to recombinant human bone morphogenetic protein 2 (BMP-2), a heparin-binding osteoinductive growth factor. Affinity electrophoresis analysis provided evidence that carboxy-methylated dextran polymers grafted with high amount of benzylamide groups (named DMCB) interact with BMP-2. The capability of such polysaccharides to potentiate the growth factor biological activity was then investigated. in vitro, DMCB dose-dependently promoted osteoblast differentiation induced by BMP-2 in C2C12 myoblasts more efficiently than heparin. A screening study provided evidence that the potentiating effects of the dextran derivatives on the BMP-2-induced alkaline phosphatase activity improved with their benzylamide groups content and, therefore, with their affinity for the growth factor. The biological activity of BMP-2 was monitored in the culture medium after 6 days using C2C12 cells (containing a BMP sensitive luciferase reporter gene). Like heparin, DMCB sustained the biological activity of the growth factor; this result suggests that the formation of the BMP-2/DMCB complex may protect the protein from being inactivated. In rats in vivo, DMCB also stimulated ectopic calcification mediated by BMP-2. These data indicate that dextran-based polysaccharides prolong the half-life of the growth factor and promote its biological activity. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 88A: 174-183, 2009
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available