GDNF blended chitosan nerve guides: An in vivo study

Chitosan nerve guides are currently being utilized to repair damaged or injured peripheral nerves. To enhance the nerve regeneration process, instead of using the material alone, researchers are focusing on blending different proteins or molecules with chitosan to facilitate nerve repair and regeneration. In our study, we have blended chitosan with glial cell-line derived neurotrophic factor and laminin within our nerve guides (GLC). The rat sciatic nerve injury model was used to test these nerve guides and histologically evaluated at 6, 9, and 12 weeks. Histologically at 6 weeks, the axon area and myelination are significantly higher in the GLC group compared with the cotrols. However, at 9 and 12 weeks control groups matched the GLC values. Thus the histological results indicate that GLC nerve guides can enhance the nerve regeneration process during the inital stages of nerve repair. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 154-165, 2009