Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 90A, Issue 1, Pages 82-93Publisher
WILEY
DOI: 10.1002/jbm.a.32060
Keywords
biomaterials; nitric oxide; inflammation; macrophage; hyaluronic acid
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Inflammatory reactions to biomaterials may include macrophage-mediated generation of nitric oxide (NO), which may harm patient tissue or potentially interfere with proper function of an implanted device. RAW 264.7 cells were grown in culture and treated at various times with lipopolysaccharide (LPS, endotoxin), murine recombinant gamma-interferon (mrIFN-gamma), and different preparation of hyaluronic acid (HA). Increase ill fluorescence of 2,3-diaminonaphthalene (DAN) allowed for detection of initial (24 h or less) NO inflammatory responses of. RAW 264.7 to LPS from E. coli O26:B6. By looking at early time points, mrIFN-gamma augmentation of the LPS effect was observed, simulating a complex immune reaction. Activation through nuclear factor-kappa B (NF-kappa B), was confirmed in this system by parthenolide inhibition of LPS stimulation.
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