Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 90A, Issue 2, Pages 506-513Publisher
WILEY
DOI: 10.1002/jbm.a.31950
Keywords
poly(epsilon-caprolactone)-Pluronic-poly(epsilon-caprolactone); cationic nanoparticles; DNA delivery; cytotoxicity; DNA degradation
Funding
- National Natural Science Foundation of China [NSFC20704027]
- Sichuan Prominent Young Talents Program [07ZQ026-033]
- Sichuan Key Project of Science and Technology [06(05SG022-021-02), 2007SGY019]
- Chinese Key Basic Research Program [2004CB51.8807]
- National High-Tech Project of China [2007AA021900]
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In this article, poly (epsilon-caprolactone)-Pluronic-poly(epsilon-caprolactone) (PCFC) copolymer was synthesized by ring-opening polymerization and characterized by H-1-NMR and GPC. Cationic PCFC nanoparticles were prepared at one-step by modified emulsion solvent evaporation method using cetyltrimethylammonium bromide as the stabilizer. The cytotoxicity of PCFC nanoparticles was studied here with and without serum. The obtained cationic PCFC nanoparticles were employed to condense and adsorb DNA onto its surface. And it Could protect plasmid GFP (pGFP) from enzymatic degradation and acidic degradation in a certain period. Release behavior of pGFP from the pGFP/PCFC nanoparticles complex was also studied in vitro. The obtained cationic PCEC nanoparticles might have great potential application in DNA delivery. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 506-513, 2009
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