Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 86A, Issue 1, Pages 31-40Publisher
WILEY
DOI: 10.1002/jbm.a.31542
Keywords
osteoblasts; Raman spectroscopy; differentiation; bioactive glass; gene expression
Funding
- Medical Research Council Funding Source: Medline
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Here, we report on a rapid, noninvasive biophotonics system using Raman spectroscopy to detect realtime biochemical changes in foetal osteoblasts (17013s) following exposure to 45S5 Bioglass (R) (BG)-conditioned media. Bio-Raman spectroscopy, combined with multivariate statistical analysis techniques (principal component analysis and least squares analysis), was able to noninvasively identify biochemical differences in FOBs cultured for different time periods and between FOBs exposed/or not to BG-conditioned media. Gene and protein expression studies were also performed for known markers of osteoblastic differentiation, namely, alkaline phosphatase, bone sialoprotein, and collagen type I. Quantitative RT-PCR confirmed upregulation of genes associated with osteoblast differentiation after exposure to BG-conditioned media. These results suggest that Raman spectroscopy can noninvasively detect biochemical changes in FOBs associated with differentiation. This technique could have important applications in the field of regenerative medicine by enabling rapid characterization of cell or organoid behavior on novel bioactive scaffolds without damage to either cell or biomaterial. (C) 2007 Wiley Periodicals, Inc.
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