Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 87A, Issue 2, Pages 364-372Publisher
WILEY
DOI: 10.1002/jbm.a.31786
Keywords
magnetic nanoparticles; glucosaminic acid; water-dispersion magnetic capture probe; cellular uptake
Funding
- National Basic Research Program of China [2005CB623903]
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A novel and simple method to form water-dispersed magnetic nanoparticles was Successfully developed through glucosaminic acid-surface modification of iron oxide nanoparticles. The resultant glucosaminic acid-modified magnetic nanoparticles (GA-MNP's) had not only good uniformity in spherical shape with diameter of about 1013 nm, but also possessed excellent water-dispersity and stability. In Cell Culture experiments, the internalization of GA-MNPs into different kinds of cells was observed over a 5-day period. The results indicated that the internalization of GA-MNPs into mouse macrophage, cells and mouse embryonic fibroblast cells was not observed after 40 h of Culturing. However, the GA-MNPs were internalized quickly into cancer cells after just 24 h Of Culturing. TEM images of the GA-MNPs uptake in ECA-109 cells were used to study the internalization mechanisms of GA-MNPs and their distribution in ECA-109 cells. Additionally, a water-dispersed magnetic capture probe was prepared by immobilization Of oligonucleotides onto GA-MNPs, and the probe was used for detection and separation of their complementary oligonucleotides sequence. (c) 2008 Wiley Periodicals, Inc.
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