Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 86A, Issue 1, Pages 278-288Publisher
WILEY-LISS
DOI: 10.1002/jbm.a.32030
Keywords
cellular microenvironment; dynamic co-culture; parylene-C stencils; cell patterning
Funding
- Directorate For Engineering
- Div Of Engineering Education and Centers [832785] Funding Source: National Science Foundation
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Co-culturing different cell types can be useful to engineer a more in vivo-like microenvironment for cells in culture. Recent approaches to generating cellular co-cultures have used microfabrication technologies to regulate the degree of cell-cell contact between different cell types. However, these approaches are often limited to the co-culture of only two cell types in static cultures. The dynamic aspect of cell-cell interaction, however, is a key regulator of many biological processes such as early development, stem cell differentiation, and tissue regeneration. In this study, we describe a micropatterning technique based on microfabricated multilayer parylene-C stencils and demonstrate the potential of parylene-C technology for co-patterning of proteins and cells with the ability to generate a series of at least five temporally controlled patterned cocultures. We generated dynamic co-cultures of murine embryonic stem cells in culture with various secondary cell types that could be sequentially introduced and removed from the co-cultures. Our studies suggested that dynamic co-cultures generated by using parylene-C stencils may be applicable in studies investigating cellular interactions in controlled microenvironments such as studies of ES cell differentiation, wound healing and development. (c) 2008 Wiley Periodicals, Inc.
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