Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 22, Issue 8, Pages 1069-1082Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1163/092050610X498204
Keywords
Electrospinning; silk fibroin-hydroxybutyl chitosan; scaffold; tissue engineering
Funding
- national high technology research and developed program (863 Program) [2008AA03Z305]
- Science and technology commission of Shanghai municipality program [08520704600, 0852 nm 03400]
- '111 Project' Biomedical textile materials science and technology [B07024]
- State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Research Program [LZ0906]
- Shanghai-Unilever Research and Development Fund [08520750100]
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Silk fibroin (SF) hydroxybutyl chitosan (HBC) blend nanofibrous scaffolds were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and trifluoroacetic acid (TFA) as solvents to biomimic the native ECM by electrospinning. SEM results showed that be average nanofibrous diameter increased when the content of HBC was raised from 20% to 100%. Whereas water contact angle measurements confirmed that SF/HBC nanofibrous scaffolds with different weight ratios were of good hydrophilicity. Both the tensile strength and the elongation at break were improved obviously when the weight ratio of SF to HBC was 20:80. C-13-NMR clarified that SF and HBC molecules existed in H-bond interactions, but HBC did not induce SF conformation to transform from random coil form to beta-sheet structure. Moreover, the use of genipin vapour not only induced conformation of SF to convert from random coil to beta-sheet structure but also acted as a cross-linking agent for SF and HBC. Cell viability studies demonstrated that SF/HBC nanofibrous scaffolds presented good cellular compatibility. Thus, electrospun SF/HBC blended nanofibres may provide an ideal biomimic tissue-engineering scaffold. (c) Koninklijke Brill NV, Leiden, 2011
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