Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 20, Issue 14, Pages 1975-1993Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1163/156856208X396074
Keywords
RGD; poly(caprolactone); bone marrow stromal cell; cell adhesion; focal adhesion kinase; surface modification
Funding
- NIH [R01 DE13608]
- Coulter Foundation
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R01DE013608] Funding Source: NIH RePORTER
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Poly(caprolactone) (PCL) is a promising biodegradable polymer for tissue engineering. However, intrinsically poor cell-adhesive properties of PCL may limit its application. In this study, the PCL film surface was modified with RGDC peptide by a chemical immobilization procedure. Furthermore, bone marrow stromal cell (BMSC) behaviors including attachment, spreading, focal adhesion formation, focal adhesion kinase (FAK) activation, apoptosis and proliferation when cultured on the modified PCL films were investigated. Our results demonstrated that PCL with RGD modification promoted initial BMSC attachment, spreading and focal adhesion formation. At a later time point (12 h), BMSC attachment on both RGD peptide-modified PCL and PCL-NH2 films significantly increased compared to untreated PCL films. Importantly, FAK phosphorylation was significantly increased only on the films with RGD-modified films, not on the PCL-NH2 films, demonstrating that PCL with RGD modification had an advantage in initiating the specific integrin-mediated signal transduction and might play an important role in the subsequent retardation in cell death and enhancement in cell proliferation. The present results provide more evidence that functionalizing PCL with RGD peptides may be a feasible way to improve the interaction between BMSC and PCL substrate, which is important in tissue engineering. (C) Koninklijke Brill NV, Leiden, 2009
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