Journal
JOURNAL OF BIOMATERIALS APPLICATIONS
Volume 29, Issue 2, Pages 243-253Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328214521846
Keywords
Bone regeneration; periodontal ligament stem cell; bone marrow mesenchymal stem cell; Bio-Oss; tissue-engineered bone; comparative study
Funding
- Tongji University [09411955100]
- Shanghai Committee of Science and Technology of China [10XD1404500]
- National Natural Science Foundation of China [81271110]
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The aim of this study was to compare the osteogenic effects of periodontal ligament stem cells (PDLSCs) versus bone marrow mesenchymal stem cells (BMMSCs) in combination with Bio-Oss scaffolds on subcutaneous and critical-size defects in the immunodeficient rat calvarium. PDLSCs and BMMSCs were obtained from the same canine donor. Twenty-four rats were randomly assigned to one of four experimental groups (n=6 each): group A (no-graft negative control), group B (Bio-Oss positive control), group C (BMMSC/Bio-Oss test group), and group D (PDLSC/Bio-Oss test group). Eight weeks post-transplantation, ectopic and in situ bone regeneration was evaluated by micro-computed tomography (mu-CT), histology, histomorphometry, and immunohistochemistry. The stem cell/Bio-Oss constructs were significantly superior to the controls in terms of their ability to promote osteogenesis (p<0.01), while the PDLSC/Bio-Oss construct tended to be superior to the BMMSC/Bio-Oss construct. Thus, engineered stem cell/Bio-Oss complexes can successfully reconstruct critical-size defects in rats, and PDLSCs and BMMSCs are both suitable as seed cells.
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