Journal
JOURNAL OF BIOMATERIALS APPLICATIONS
Volume 29, Issue 8, Pages 1155-1166Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328214553959
Keywords
Scaffolds; chitosan; xanthan gum; stem cells; dressings; regenerative medicine; skin lesions
Funding
- Sao Paulo Research Foundation (FAPESP, Brazil)
- Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil)
- National Council for Scientificand Technological Development (CNPq, Brazil)
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The association between tridimensional scaffolds to cells of interest has provided excellent perspectives for obtaining viable complex tissues invitro, such as skin, resulting in impressive advances in the field of tissue engineering applied to regenerative therapies. The use of multipotent mesenchymal stromal cells in the treatment of dermo-epidermal wounds is particularly promising due to several relevant properties of these cells, such as high capacity of proliferation in culture, potential of differentiation in multiple skin cell types, important paracrine and immunomodulatory effects, among others. Membranes of chitosan complexed with xanthan may be potentially useful as scaffolds for multipotent mesenchymal stromal cells, given that they present suitable physico-chemical characteristics and have adequate tridimensional structure for the adhesion, growth, and maintenance of cell function. Therefore, the purpose of this work was to assess the applicability of bioactive dressings associating dense and porous chitosan-xanthan membranes to multipotent mesenchymal stromal cells for the treatment of skin wounds. The membranes showed to be non-mutagenic and allowed efficient adhesion and proliferation of the mesenchymal stromal cells invitro. Invivo assays performed with mesenchymal stromal cells grown on the surface of the dense membranes showed acceleration of wound healing in Wistar rats, thus indicating that the use of this cell-scaffold association for tissue engineering purposes is feasible and attractive.
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