Journal
JOURNAL OF BIOMATERIALS APPLICATIONS
Volume 27, Issue 7, Pages 862-871Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328211428094
Keywords
Osteogenesis; micropatterning; photolithography; biocompatibility; human mesenchymal stem cells
Funding
- Finska Lakaresallskapet
- National Graduate School of Musculoskeletal Diseases and Biomaterials
- Orion Farmos Research Foundation
- European Science Foundation
- Regenerative medicine, and Danish Council for Strategic Research `Individualized Musculoskeletal Medicine
- Grants-in-Aid for Scientific Research [24791522, 24592252, 21591929] Funding Source: KAKEN
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Osteogenic responses of human mesenchymal stromal cells (hMSCs) were compared on square-patterned, inverse square-patterned, and planar titanium, chromium, diamond-like carbon (DLC), and tantalum; hypothesis was that both the materials and patterns affect osteogenesis. Samples were produced using photolithography and physical vapor deposition. Early-marker alkaline phosphatase (ALP) and mid-markers, small body size and mothers against decapentaplegic-related protein-1 (SMAD1), runt-related transcription factor-2 (RUNX2), and osteopontin were studied using quantitative real-time polymerase chain reaction. ALP and hydroxyapatite, were colorimetrically studied. ALP reached highest values on both patterned titanium samples, but mid-markers disclosed that it was already lagging behind planar and inverse patterned tantalum. Hydroxyapatite formation disclosed that osteo-induced hMSCs passed all the differentiation stages (except on planar chromium). Presence of hydroxyapatite disclosed that both types of patterning promoted (p < 0.001) osteogenesis compared to planar samples. Results suggest that the osseocompatibility/integration of implants could be improved by changing the monotonous and featureless implant-host interface into micro-patterned interface to provide physical differentiation cues.
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