Journal
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Volume 14, Issue 4, Pages 507-519Publisher
SPRINGER
DOI: 10.1007/s00775-009-0466-z
Keywords
Auranofin; Selenium; Extended X-ray absorption fine structure; Clostridium difficile; Antimicrobial
Funding
- National Institutes of Health [GM042025, ES01434]
- Department of Energy
- Office of Biological and Environmental Research
- National Center for Research Resources
- Biomedical Technology Program
- Florida Department of Health [05-NIR-10]
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Clostridium difficile is a nosocomial pathogen whose incidence and importance are on the rise. Previous work in our laboratory characterized the central role of selenoenzyme-dependent Stickland reactions in C. difficile metabolism. In this work we have identified, using mass spectrometry, a stable complex formed upon reaction of auranofin (a gold-containing drug) with selenide in vitro. X-ray absorption spectroscopy supports the structure that we proposed on the basis of mass-spectrometric data. Auranofin potently inhibits the growth of C. difficile but does not similarly affect other clostridia that do not utilize selenoproteins to obtain energy. Moreover, auranofin inhibits the incorporation of radioisotope selenium (Se-75) in selenoproteins in both Escherichia coli, the prokaryotic model for selenoprotein synthesis, and C. difficile without impacting total protein synthesis. Auranofin blocks the uptake of selenium and results in the accumulation of the auranofin-selenide adduct in the culture medium. Addition of selenium in the form of selenite or L-selenocysteine to the growth medium significantly reduces the inhibitory action of auranofin on the growth of C. difficile. On the basis of these results, we propose that formation of this complex and the subsequent deficiency in available selenium for selenoprotein synthesis is the mechanism by which auranofin inhibits C. difficile growth. This study demonstrates that targeting selenium metabolism provides a new avenue for antimicrobial development against C. difficile and other selenium-dependent pathogens.
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