Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 289, Issue 32, Pages 22258-22267Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M114.588046
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Funding
- National Basic Research Program of China (973 Program) [2013CB932902]
- NSFC [61071047, 31371003]
- Natural Science Foundation of Jiangsu Province [BK2012122, BK20130886]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20130092110030]
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Exosomes are nanoscale membrane vesicles secreted from many types of cells. Carrying functional molecules, exosomes transfer information between cells and mediate many physiological and pathological processes. In this report, utilizing selective inhibitors, molecular tools, and specific endocytosis markers, the cellular uptake of PC12 cell-derived exosomes was imaged by high-throughput microscopy and statistically analyzed. It was found that the uptake was through clathrin-mediated endocytosis and macropinocytosis. Furthermore, PC12 cell-derived exosomes can enter and deliver microRNAs (miRNAs) into bone marrow-derived mesenchymal stromal cells (BMSCs), and decrease the expression level of transforming growth factor beta receptor II (TGF beta RII) and tropomyosin-1 (TPM1) through miR-21. These results show the pathway of exosome internalization and demonstrate that tumor cell-derived exosomes regulate target gene expression in normal cells.
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