Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 289, Issue 10, Pages 6604-6618Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M114.549758
Keywords
Bone Morphogenetic Protein (BMP); Development; Gene Regulation; SMAD Transcription Factor; Zebrafish; Dorso-ventral Patterning; Myelopoiesis
Categories
Funding
- China 973 Project [2010CB126306, 2012CB944504]
- National Natural Science Foundation of China (NSFC) [31222052]
- NSFC [30771100]
- FEBL Grant [2011FBZ23]
Ask authors/readers for more resources
Background: Ventral specification is regulated by Smad1-, Smad5-, and/or Smad9-mediated bone morphogenetic protein (BMP) signaling. Results: Double knockdown instead of single knockdown of smad1 and smad9 induces dorsalization, which cannot be rescued by smad5 overexpression. Conclusion:smad1 and smad9 act redundantly and downstream of smad5 to mediate ventral specification. Significance: The regulation network and cooperative roles of BMP R-Smads in early development are clarified. Bone morphogenetic proteins (BMPs) are multifunctional growth factors that play crucial roles during embryonic development and cell fate determination. Nuclear transduction of BMP signals requires the receptor type Smad proteins, Smad1, Smad5, and Smad9. However, how these Smad proteins cooperate in vivo to regulate various developmental processes is largely unknown. In zebrafish, it was widely believed that the maternally expressed smad5 is essential for dorso-ventral (DV) patterning, and the zygotically transcribed smad1 is not required for normal DV axis establishment. In the present study, we have identified zygotically expressed smad9, which cooperates with smad1 downstream of smad5, to mediate zebrafish early DV patterning in a functional redundant manner. Although knockdown of smad1 or smad9 alone does not lead to visible dorsalization, double knockdown strongly dorsalizes zebrafish embryos, which cannot be efficiently rescued by smad5 overexpression, whereas the dorsalization induced by smad5 knockdown can be fully rescued by overexpression of smad1 or smad9. We have further revealed that the transcription initiations of smad1 and smad9 are repressed by each other, that they are direct transcriptional targets of Smad5, and that smad9, like smad1, is required for myelopoiesis. In conclusion, our study uncovers that smad1 and smad9 act redundantly to each other downstream of smad5 to mediate ventral specification and to regulate embryonic myelopoiesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available