Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 288, Issue 39, Pages 27972-27985Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.477547
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Funding
- Italian Ministry of Health [RF-2009-1474624]
- University of Bologna [RFO 2008-2010]
- Gino Galletti Foundation
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Prion diseases are characterized by tissue accumulation of a misfolded, beta-sheet-enriched isoform (scrapie prion protein (PrPSc)) of the cellular prion protein (PrPC). At variance with PrPC, PrPSc shows a partial resistance to protease digestion and forms highly aggregated and detergent-insoluble polymers, two properties that have been consistently used to distinguish the two proteins. In recent years, however, the idea that PrPSc itself comprises heterogeneous species has grown. Most importantly, a putative proteinase K (PK)-sensitive form of PrPSc (sPrP(Sc)) is being increasingly investigated for its possible role in prion infectivity, neurotoxicity, and strain variability. The study of sPrP(Sc), however, remains technically challenging because of the need of separating it from PrPC without using proteases. In this study, we have systematically analyzed both PK resistance and the aggregation state of purified PrPSc across the whole spectrum of the currently characterized human prion strains. The results show that PrPSc isolates manifest significant strain-specific differences in their PK digestion profile that are only partially explained by differences in the size of aggregates, suggesting that other factors, likely acting on PrPSc aggregate stability, determine its resistance to proteolysis. Fully protease-sensitive low molecular weight aggregates were detected in all isolates but in a limited proportion of the overall PrPSc (i.e. <10%), arguing against a significant role of slowly sedimenting PK-sensitive PrPSc in the biogenesis of prion strains. Finally, we highlight the limitations of current operational definitions of sPrP(Sc) and of the quantitative analytical measurements that are not based on the isolation of a fully PK-sensitive PrPSc form.
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