Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 288, Issue 21, Pages 15085-15097Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.448837
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Core Research for Evolutional Science and Technology (CREST) program of Japan Science and Technology Agency (JST)
- Grants-in-Aid for Scientific Research [23570228, 23241063, 24657135] Funding Source: KAKEN
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Oct4 is a member of the POU family of transcription factors and plays a critical role in both maintenance of the undifferentiated state of embryonic stem (ES) cells and in the reprogramming of somatic cells to induced pluripotent stem cells. Oct4 is imported into the nucleus where it functions as a transcription factor; however, the spatiotemporal dynamic behavior of Oct4 remains largely unknown. In the present study we show that Oct4 is a nucleocytoplasmic shuttling protein. Furthermore, although Oct4 mutants with altered nuclear import/export activity were able to maintain the self-renewal of ES cells, they displayed limited potential for cellular reprogramming. These results indicate that the intracellular localization of Oct4, which is dependent on nucleocytoplasmic shuttling, must be more strictly regulated for cellular reprogramming, suggesting that Oct4 plays differential roles in the self-renewal of ES cells and in somatic cell reprogramming.
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