4.6 Article

Extracellular Signal-regulated Kinase (ERK) Phosphorylates Histone Deacetylase 6 (HDAC6) at Serine 1035 to Stimulate Cell Migration

Journal

JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 288, Issue 46, Pages 33156-33170

Publisher

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.472506

Keywords

Cell Migration; ERK; Histone Deacetylase; MAP Kinases (MAPKs); Protein Phosphorylation

Funding

  1. National Institutes of Health Grant from the NCI [R01CA164147]
  2. James Esther King Biomedical Research Program [09KN-17]
  3. a Moffitt Lung SPORE career development grant
  4. Ovarian Cancer Research Fund
  5. University of South Florida graduate student success diversity fellowship

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Background: HDAC6 plays an important role in cell migration. Results: ERK interacts with and phosphorylates HDAC6 to promote cell migration. Conclusion: ERK signaling pathway promotes cell migration, in part, through phosphorylating HDAC6. Significance: Inhibition of HDAC6 activity as well as the EGFR-Ras-Raf-MEK-ERK signaling pathway may cooperatively reduce cell migration. Histone deacetylase 6 (HDAC6) is well known for its ability to promote cell migration through deacetylation of its cytoplasmic substrates such as -tubulin. However, how HDAC6 itself is regulated to control cell motility remains elusive. Previous studies have shown that one third of extracellular signal-regulated kinase (ERK) is associated with the microtubule cytoskeleton in cells. Yet, no connection between HDAC6 and ERK has been discovered. Here, for the first time, we reveal that ERK binds to and phosphorylates HDAC6 to promote cell migration via deacetylation of -tubulin. We have identified two novel ERK-mediated phosphorylation sites: threonine 1031 and serine 1035 in HDAC6. Both sites were phosphorylated by ERK1 in vitro, whereas Ser-1035 was phosphorylated in response to the activation of EGFR-Ras-Raf-MEK-ERK signaling pathway in vivo. HDAC6-null mouse embryonic fibroblasts rescued by the nonphosphorylation mimicking mutant displayed significantly reduced cell migration compared with those rescued by the wild type. Consistently, the nonphosphorylation mimicking mutant exerted lower tubulin deacetylase activity in vivo compared with the wild type. These data indicate that ERK/HDAC6-mediated cell motility is through deacetylation of -tubulin. Overall, our results suggest that HDAC6-mediated cell migration could be governed by EGFR-Ras-Raf-MEK-ERK signaling.

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