Metabolic Flux of Extracellular Heme Uptake in Pseudomonas aeruginosa Is Driven by the Iron-regulated Heme Oxygenase (HemO)

Heme utilization by Pseudomonas aeruginosa involves several proteins required for internalization and degradation of heme. In the following report we provide the first direct in vivo evidence for the specific degradation of extracellular heme to biliverdin (BV) by the iron-regulated HemO. Moreover, through isotopic labeling (C-13-heme) and electrospray ionization-MS analysis we have confirmed the regioselectivity and ratio of C-13-delta and beta-BV IX (70:30) is identical in vivo to that previously observed for the purified protein. Furthermore, the C-13-BV IX delta and BV IX beta products are effluxed from the cell by an as yet unidentified transporter. Conversion of extracellular heme to BV is dependent solely on the iron-regulated HemO as evidenced by the lack of BV production in the P. aeruginosa hemO deletion strain. Complementation of P. aeruginosa Delta hemO with a plasmid expressing either the wild type HemO or alpha-regioselective HemO mutant restored extracellular heme uptake and degradation. In contrast deletion of the gene encoding the cytoplasmic heme-binding protein, PhuS, homologs of which have been proposed to be heme oxygenases, did not eliminate C-13-BV IX delta and IX beta production. In conclusion the metabolic flux of extracellular heme as a source of iron is driven by the catalytic action of HemO.