Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 287, Issue 23, Pages 19242-19254Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.349936
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- National Natural Science Foundation of China [30930083]
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Human MutS homologue 2 (hMSH2), a crucial element of the highly conserved DNA mismatch repair system, maintains genetic stability in the nucleus of normal cells. Our previous studies indicate that hMSH2 is ectopically expressed on the surface of epithelial tumor cells and recognized by both T cell receptor gamma delta (TCR gamma delta) and natural killer group 2 member D (NKG2D) on V delta 2 T cells. Ectopically expressed hMSH2 could trigger a gamma delta T cell-mediated cytolysis. In this study, we showed that oxidative stress induced ectopic expression of hMSH2 on human renal carcinoma cells. Under oxidative stress, both p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) pathways have been confirmed to mediate the ectopic expression of hMSH2 through the apoptosis-signaling kinase 1 (ASK1) upstream and activating transcription factor 3 (ATF3) downstream of both pathways. Moreover, renal carcinoma cell-derived interleukin (IL)-18 in oxidative stress was a prominent stimulator for ectopically induced expression of hMSH2, which was promoted by interferon (IFN)-gamma as well. Finally, oxidative stress or pretreatment with IL-18 and IFN-gamma enhanced gamma delta T cell-mediated cytolysis of renal carcinoma cells. Our results not only establish a mechanism of ectopic hMSH2 expression in tumor cells but also find a biological linkage between ectopic expression of hMSH2 and activation of gamma delta T cells in stressful conditions. Because gamma delta T cells play an important role in the early stage of innate anti-tumor response, gamma delta T cell activation triggered by ectopically expressed hMSH2 may be an important event in immunosurveillance for carcinogenesis.
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