Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 287, Issue 37, Pages 31457-31461Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.C112.397059
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Funding
- Wellcome Trust
- Medical Research Council
- MRC [G0001352, G0400106] Funding Source: UKRI
- Medical Research Council [G0400106, G1000758, MR/J006742/1, G1000758B, G0001352] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0508-10212] Funding Source: researchfish
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IgE, the antibody that mediates allergic responses, acts as part of a self-regulating protein network. Its unique effector functions are controlled through interactions of its Fc region with two cellular receptors, Fc epsilon RI on mast cells and basophils and CD23 on B cells. IgE cross-linked by allergen triggers mast cell activation via Fc epsilon RI, whereas IgE-CD23 interactions control IgE expression levels. We have determined the CD23 binding site on IgE, using a combination of NMR chemical shift mapping and site-directed mutagenesis. We show that the CD23 and Fc epsilon RI interaction sites are at opposite ends of the C epsilon 3 domain of IgE, but that receptor binding is mutually inhibitory, mediated by an allosteric mechanism. This prevents CD23-mediated cross-linking of IgE bound to Fc epsilon RI on mast cells and resulting antigen-independent anaphylaxis. The mutually inhibitory nature of receptor binding provides a degree of autonomy for the individual activities mediated by IgE-Fc epsilon RI and IgE-CD23 interactions.
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